Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 7 - Jul 12
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse PAI1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Plasminogen Activator Inhibitor 1 (PAI1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Plasminogen Activator Inhibitor 1 (PAI1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Plasminogen Activator Inhibitor 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | PAI-1, also known as endothelial prothrombin activator inhibitor or serum protein E1, is a serine protease inhibitor (serpin). Its primary function is to inhibit urokinase plasmakinase activator (uPA), an enzyme responsible for cleaving plasmakinogen to form plasmakin. Plasmin, either by itself or in conjunction with matrix metalloproteinases, mediates the degradation of the extracellular matrix. In this context, PAI-1 inhibits uPA by binding to its active site, preventing plasmakin formation. Additional inhibition occurs through PAI-1 binding to the uPA/uPA receptor complex, leading to its degradation. Therefore, PAI inhibits the serine proteases tPA and uPA/urokinase, thereby acting as an inhibitor of fibrinolysis, the physiological process that degrades blood clots. Furthermore, PAI-1 inhibits the activity of matrix metalloproteinases, which play a key role in the invasion of malignant cells through the basal lamina. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.6 ★★★★★
Based on 392 reviews
Sort
Product Reviews
★★★★★ 3
No me sirvio
No cumplió para lo que fue comprado
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 9, 2023
★★★★★ 5
Good product
Good product
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 25, 2025
★★★★★ 5
High-Quality Omega 3 – Easy to Take, No Fishy Aftertaste
Size: 90 Count (Pack of 1)
I’ve been using Qunol’s Advanced Omega 3 Krill and Fish Oil Complex, and it’s one of the better omega supplements I’ve tried. The capsules are easy to swallow, and I haven’t experienced any fishy burps or aftertaste. The blend of krill and fish oil feels well-formulated, and the claim of better bioavailability makes sense since I notice benefits with fewer capsules compared to standard fish oil.
I like that it combines the strengths of both krill and fish oil—EPA and DHA support heart, brain, and joint health, while the added phospholipids from krill oil may improve absorption. The softgels are also smaller and easier to take than many large fish oil pills.
Pros:
Combines krill and fish oil for balanced omega support
No fishy aftertaste or burps
Smaller, easy-to-swallow softgels
Formulated for better absorption
Cons:
Pricier than standard fish oil supplements
Bottle size could be larger for daily users
Overall, this is a premium omega 3 supplement that’s worth it if you want high absorption and a cleaner experience. A reliable option for long-term heart and brain support.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 3, 2025
★★★★★ 5
Wonder
Size: 90 Count (Pack of 1)
Qunol Vitamins Review
Qunol is a well-regarded brand in the supplement industry, known for its high-quality vitamins and supplements that support various aspects of health. Here’s a detailed look at what makes Qunol Vitamins a popular choice:
Effectiveness:
Qunol Vitamins are praised for their effectiveness in supporting overall health and well-being. The brand offers a range of products, including CoQ10, turmeric, and magnesium supplements, each designed to address specific health needs. Users often report noticeable improvements in energy levels, cardiovascular health, and joint comfortAd12.
Ingredients:
Qunol products are formulated with high-quality, bioavailable ingredients. For example, Qunol Ultra CoQ10 is known for its superior absorption compared to regular CoQ10 supplements, thanks to its water and fat-soluble formulation3. The turmeric supplements contain a high concentration of curcuminoids, which are the active compounds known for their anti-inflammatory propertiesAd1.
Taste and Ease of Use:
Many Qunol supplements come in easy-to-swallow softgels or capsules, making them convenient to incorporate into daily routines. Some products, like the liquid CoQ10, have a pleasant taste, which is a bonus for those who prefer liquid supplements3.
Packaging and Price:
Qunol products are well-packaged to ensure freshness and potency. The pricing is competitive, especially considering the quality and effectiveness of the ingredients used. While they may be slightly more expensive than some other brands, many users find the benefits justify the costAd13.
Overall Impression:
Qunol Vitamins stand out for their high-quality formulations and proven effectiveness. Whether you’re looking to boost your cardiovascular health, reduce inflammation, or support overall wellness, Qunol offers reliable and effective options. The brand’s commitment to using bioavailable ingredients ensures that you get the most out of each supplementAd123.
I hope this helps! Let me know if you need any more information.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 2, 2024
★★★★★ 5
One of my favorites!
Size: 60 Count (Pack of 1)
It’s a very big bottle and has been my favorite for several months now. A lot of fish oil pills tend to be very uncomfortable to swallow but not these. I can swallow the single serving (2 capsules) at once which is not the case for most fish oil pills out there. I think these are definitely worth the money. Has definitely helped with my pain relief the past few months after being recommended to start taking this. I haven’t noticed too much in my skin health to be completely honest, i do a lot of other things with my skin and have a strict skincare routine already so i don’t think i would even really notice. I recently learned that fish can improve Skin health so it’s something i’ll look out for. The quality of the pills are great i’ve never once had one explode. Overall these are very effective. And they definitely affect your energy level in a positive way.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 16, 2025